Evaluation of Candida albicans adhesion on AlSl-316L and Ti6Al4V in changing environmental conditions

Currently, studies on the formation of microbiological biofilm on biomaterial surfaces and medical devices have become an important aspect in medicine. In vivo studies of the biofilm development show a high correlation between the environmental factors and attachment of microorganisms to artificial surfaces. This paper is devoted to determining the impact of specific environmental stimuli and parameters of cultivation of Candida albicans yeasts on their adhesion to AISI-316L and Ti6Al4V substrates. Environmental parameters include variable glucose concentrations in YPG growth medium (0, 2, 4 and 10% wt.). Development of yeasts was also analyzed in terms of variable nutrients level i.e. transferring samples from medium with low nutrients level to other with higher glucose content. Another studied parameter was incubation time and how much fungal colonization varies during 24 and 48 h of incubation. For all samples, the number of both live and dead cells was taken into account. The results showed that with the increase in sugar content in culture media, biofilm development was observed, especially on AISI 316L surface. Also, significant changes in the number of adhered cells were observed with higher glucose concentration in medium after 24 h of incubation. Almost similar number of attached cells was observed for low glucose concentrations on both studied materials.