Histochemiczne badania aktywności dehydrogenazy mleczanowej w układzie Fasciola hepatica-Mus musculus

A histochemical assay was elaborated to study lactate dehydrogenase activity in the tissues of Fasciola hepatica and in the liver of mice during the course of fasciolosis. LDH activity was determined in the tegument, muscles, parenchyma, oral sucker and the caecum of juvenile and adult liver flukes inhabiting the host liver parenchyma and bile ducts. The most substantial changes in LDH activity were recorded in the tegument outer surface layer. White mice of CFW strain were infected with 10, 30 and 50 doses of F. hepatica metacerkariae administered per os. A dramatic decrease in LDH activity in the experimental mice liver during the course of infection, particularly in the acute fasciolosis was observed.