Antimicrobial resistant and virulence genes profiles of some Gram-negative bacteria from clinical isolates at Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria
Antimicrobial resistant and virulence genes profiles of some Gram-negative bacteria from clinical isolates at Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria
Introduction and objective. Infections due to multidrug-resistant (MDR) Enterobacteriaceae are an ongoing global threat
in their management. The aim of the study was to investigate the antiimicrobial resistance (AMR) and virulence gene profiles
of MDR Gram-negative isolates in Sokoto, north-west Nigeria.
Materials and method. A total of 578 clinical samples were collected from patients. Suspected Gram-negative bacteria
were isolated from these clinical samples: vaginal swab, pus, stool, blood, wound swab and urine, using Gram-staining and
conventional biochemical reactions. These isolates were further identified with an identification kit (Microgen-GN-A), and
tested against a panel of 11 antibiotics. A single polymerase chain reaction (PCR) assay targeting 13 virulence gene related
to adhesion (fimH, papC, and sfaS), iron chelation (iutA, and fyuA), toxins (astA, stx1, stx2, and eaeA), biofilm (bssS), and serum
resistance (traT, iss, and kapsMTII) encoding genes were evaluated.
Results. A total of 276 Gram-negative isolates were identified using the Gram stain and biochemical reactions. These
organisms were further confirmed with identification kit. Of the 276 isolates, 36 organisms of interest (23 Escherichia coli,
4 Klebsiella pneumoniae and 9 Proteus mirabilis) were identified. Other Gram-negative isolates accounted for the remaining
86.9%. The majority of the isolates were resistant to cefixime (100%) and partially resistant to amikacin (19.4%).The virulence
genes bssS (58.3%), fimH (44.4%), and iutA (44.4%) were the most prevalent, whereas kapsMTII (5.6%) and stx2 (2.8%) were
least detected, while astA was not detected in any of the isolates.
Conclusion. The study elucidated the prevalence of antibiotic resistance and virulence genes in Gram-negative bacteria from
clinical isolates in Sokoto, north-western Nigeria. The majority of the isolates were MDR, thereby posing a public health risk.
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