Introduction and objective. Young green barley is the most valuable source of nutrients and bioactive substances. It has
a broad spectrum of health-promoting properties such as antioxidant, anti-inflammatory, hypoglycaemic, anti-depressant,
anti-atherosclerotic and anticancer. The presented study is an attempt to extend this knowledge with particular emphasis
on the possibility of using green barley in colon cancer prevention.
Materials and method. Extracts were prepared on the basis of two commercial products: ground dried barley grass (YGB
INT) and powder of young green barley juice (YGB GW). Their influence on colon epithelial cells (CCD841 CoN) viability
and proliferation were analyzed by LDH and MTT assays. Anticancer properties of extracts were screened on colon cancer
cell lines (LS180, HT-29) by MTT and BrdU assays. Changes in cells morphology induced by extracts were investigated after
May-Grünwald-Giemsa staining.
Results. Tested extracts were not toxic against CCD841 CoN and did not affected their proliferation or morphology (LDH
test, MTT test, microscopy observation). The MTT revealed that extracts significantly inhibited proliferation of colon cancer
cells in a dose-dependent manner. Results of BrdU test confirmed antiproliferative properties of extracts, but opposite to
MTT test, indicated YGB GW as a better anticancer agent. Light microscopy observation proved the data obtained from
both MTT and BrdU tests and additionally suggested the ability of the extracts to induce necrosis in LS180 and HT-29 cells.
Conclusions. The study demonstrated that YGB extracts specifically inhibit proliferation of colon cancer cells without any
undesirable effect on colon epithelial cells. Obtained results will provide a rationele for the future development of dietary
supplements which could be beneficial in colon cancer chemoprevention.
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