Concerning the contribution of fungi to soil carbon
sequestration, various methods have been used to extract ergosterol
from soil samples. This study aims to explore the extraction
ability and applicability of commonly used methods to extract
ergosterol from two contrasting soils. An agricultural soil (chernozem)
and a forest soil (podzol) were extracted with different
types of cell lysis such as alkaline, glass bead, and ultrasonication
methods in association with simple shaking. The ergosterol concentration
was measured by high pressure liquid chromatography.
Regardless of the method applied, ergosterol yield was higher
in podzol than in chernozem. Alkaline extraction resulted in the
highest ergosterol concentrations for both soils and miniaturized
glass bead extraction produced comparable results in the case of
chernozem. In terms of applicability, the non-alkaline methods
were simpler to conduct and less demanding of labour, chemical
use and glassware and more flexible in terms of the equipment
used for mechanical disruption. Despite the limit of the two soil
types in the present study, only the simple shaking method was
revealed to be dependent on soil type. Based on our results, we
recommend the miniaturized glass bead method for agricultural
soils, low in organic matter for high throughput. However, not all
of the methods described allow for the proper separation of coextracted
organic substances from organic-rich soil.
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