Single-Bond Force Measured by Means of Scanning Force MicroscopyD

The aim of this investigation was to determine the adhesion force as a function of calcium dichloride concentration in water. The studies were performed with two surfaces carrying opposite charge in water solution: mica - as a negatively charged surface and glass covered with poly-L-lysine - as a positively charged surface, the latter due to the presence of amino groups. The surfaces were immersed in a CaCl$\text{}_{2}$ solution in the range of concentration varied from 0 to 100 mM. The scanning force microscopy was applied to determine the adhesion force by measurements of the pull-off force. Additionally, the values of the single bond force were estimated basing on the Poisson distribution of the number of binding sites.