This is the first study to report an efficient anther culture (AC) method for spelt wheat, which has an increasing
importance not only in applied research but also in organic farming and changing nutritional standards. In this
study, an efficient AC protocol has been described for ‘GK Fehér’ spelt wheat. The number of AC-derived embryolike
structures (ELS) was 62.2/100 anthers, from which we were able to regenerate 30.6 green plantlets per
100 anthers. The percentage of green plantlets production was 89.0% among the regenerated plantlets, while the
phenomenon of albinism was restricted (3.8/100 anthers). Altogether, from AC of ‘GK Fehér’ 306 green plantlets
were produced in vitro and 241 plants were acclimatized to the greenhouse conditions. Based on ploidy level
analyses, 83 spontaneous doubled haploid (DH) plants were produced (8.3 DH plants/100 anthers), so the percentage
of spontaneous rediploidization was 34.4%. The spontaneous DH plants produced fertile spikes, while
a few seeds were harvested from seven partially fertile plants.
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