The usefulness of RAPD and AFLP markers for determining genetic similarity in rye [Secale L.] species and subspecies

In this work we searched for genetic similarities in twelve wild rye species and subspecies and a control (S. cereale ssp. cereale, cv. Walet), using RAPD and AFLP markers. AFLP is useful for distinguishing homo- and heterozygotes but is not recommended for evaluation of codominant markers. We assessed the usefulness of the applied methods for examining genetic similarity in rye. RAPD yielded four groups of genetic similarity, with similarity values between 0.32 and 0.81. AFLP markers distinguished two groups of genetic similarity, ranging from 0.49 to 0.79.