Mucuna pruriens-seed extract stimulating immune system in Litopenaeus vannamei against Vibrio harveyi

Objective: To evaluate the immunostimulant (ISt) role of Mucuna pruriens-seed (Mp-seed) enriched with Artemia (Ar) in Litopenaeus vannamei (L. vannamei) infected by Vibrio harveyi (V. harveyi). Method: Fifteen shrimp (30±5g) were stocked per 250 L tank in 3 replicates and fed twice daily with Ar enriched with Mp-seed methanolic extracts at 100 ppm and 200 ppm for 21 days. Infected L. vannamei shows altered immunoparameter (IPM) and antioxidant enzyme status due to the V. harveyi infection induced stress. Results: Significantly increased (p<0.05) total haemocyte count, respiratory burst, phenoloxidase and superoxide dismutase activities were observed in shrimp fed with Mp enriched Ar diets compared to infected group. Supplementation of Mp enriched Ar in diet could enhance the immunity of shrimp against V. harveyi infection by reverses the antioxidant level. The histology of hepatopancreas (HPC) also shows Mp-fed groups has restores the normal structure. Conclusion: The study demonstrated that Mp enriched Ar in diet have a potentially role in ISt and an effective antioxidant by regulating infectious stress against vibriosis.