Mössbauer spectroscopy is not routinely used for the determination of the concentration of iron.
However, as this method does not need any pre-treatment of samples before measurements, it may be of extreme
importance for the assessment of iron in samples, which can then be used for further investigations. Biological
samples are a good example, however, as the concentrations of iron are very low in these, it is important to exclude
possible artefacts from the background spectrum related to iron present in the counter and cryostat windows.
The aim of this study was to compare two methods of determination of the amounts of iron in investigated
sample: one, in which the background spectrum was subtracted from the sample spectrum measured, and the
other, in which the obtained non-elaborated spectrum was fitted with two doublets – a doublet for the measured
sample and a doublet for the background spectrum. Three samples containing known amounts of natural iron
(400, 800 and 1600 µg) and a sample of lyophilized human brain tissue obtained from globus pallidus were assessed.
Both methods led to the creation of a very good calibration curve with a correlation coefficient of 0.99.
Although both methods gave similar results for the concentration of iron in the sample, the subtraction of the
background spectrum had a significantly lower error of the final result.
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