Effects of dietary vegetable oil and fish oil on free and bound malondialdehyde content in rat tissues

lt was studied the effects of diets supplemented with soybean oil and mackerel oil administered by gastric intubation either separately or in combination (18.5:5; 8:15.5; 3:20.5 v/v) in the amount of 0.5 ml/day for 5 weeks on the content of free and protein-bound malondialdehyde (MDA) in rat tissues. The quantity of free and bound MDA was measured by high performance liquid chromatography (HPLC) in the liver, kidney and brain tissue and by spectrophotometry in the heart and Jung tissue. Apart from the influence of dietary fats on MDA generation, the effect of benzo-{a) pyrene administered in the diet was investigated. The study demonstrated that changes in MDA concentrations were related to the kind of dietary fat, i.e. the content of fatty acids characteristic of particular fat. Serum CI-tocopherol levels declined with the increased amounts of dietary polyunsaturated fatty acids (PUF A), thereby suggesting enhanced demand for vitamin E with increased PUF A consumption.