Impact of various 2, 4-D concentrations and different vitamin mixtures on in vitro culture of coconut (Cocos nucifera L.) by utilizing seedlings shoot tip

In this experiment, seedlings as sources for shoot tips was assembled from the coconut research institute (CRI) of Chinese Academy of tropical agricultural sciences germplasm, Wenchang, Hainan, China. The shoot tips were cultured on Y3 medium to study the impact of ten concentrations of 2, 4-D and three vitamin mixtures on survival, death and development % on in vitro culture establishment stage of coconut (Cocos nucifera L.). The obtained results revealed that all 2,4-D concentrations 4,7, 75, 100, 120 and 140 mg/L + any vitamin mixture did not give any development and high concentrations of 2, 4-D increased the death %. In any case, it can be concluded that the rate of differences in survival, death and development percentages exhibited by 2, 4-D concentrations was more pronounced than the analogous ones resulted by vitamin mixtures. At any rate, utilizing of (2, 4-D at concentration 25 mg/L+ vitamin mixture 1 or 2) exhibited the greatest values of survival percentage and reduced death percentage. Just as, increased the percentages of development in in vitro culture establishment stage of coconut (Cocos nucifera L.) through seedlings shoot tip.