Effects of nitrogen fertilizers with two different inhibitors (urease and nitrification) on the survival and activity of earthworms (Octodrilus complanatus)

Earthworms are among the most important species of the soil macrofauna. However, they are very sensitive to inorganic fertilization, more specifically to urea, which makes them a well-known bio-indicator. The objective of this study was to test if a fertilizer with inhibitors could be less destructive to earthworms than urea. In two consecutive years, we studied how new type fertilizers with urea (urea + urease inhibitor, urea + nitrification inhibitor and urea + urease inhibitor + nitrification inhibitor) can differ from urea applied alone and be closer to control (no fertilizer) in terms of the impact on earthworms, verified on different days after application. The measurements concerned earthworms’ mortality, casts and weight. Moreover, some soil properties which are affected by earthworms’ activity, such as exchangeable Ca, cation exchange capacity (CEC) and soil CO2 respiration, were studied. The inhibitors that were used comprised nitrification inhibitor, dicyandiamide (DCD), urease inhibitor, N-(n-butyl) thiophosphoric triamide (NBPT), and double inhibitors, both urease and nitrification inhibitor. The study revealed how a fertilizer with inhibitors could be less destructive to earthworms than urea. Urea with urease inhibitor and urea with urease inhibitor and nitrification inhibitor had the least negative impact on earthworms’ activities. In contrast, urea and urea with nitrification inhibitor caused higher mortality among earthworms than the other fertilizers used.