Influence of micropropagation with addition of kinetin on development of a willow (Salix viminalis L.)

The aim of the dissertation was to determine (select) the best growth medium for a basket willow (Salix viminalis L.) which contains optimal concentration of kinetin. The plant material taken from three varieties of willows: Bjor, Jorr and Tora, which grew in the experimental field, was sterilized and placed on a complete MS (Murashige and Skoog, 1962) growth medium. The propagated plants were used in an experiment, where MS substrate was enriched by growth regulator – kinetin, in various concentrations (0.5 mg∙dm-3, 1 mg∙dm-3, 2 mg∙dm-3 and 3 mg∙dm-3). The substrate which contained 0.5 KIN was considered to be the best for willow’s propagation, because plants cultivated with usage of this medium have shown the largest mass, the highest number of shoots and leaves, and also the highest quantitative and elongation growth of the roots. Among examined varieties of Salix, the best one was Bjor variety, where evaluated parameters were distinctive in comparison to two remaining varieties.