Presence of CL influence the quantity and quality of COC in slaughter house derived ovaries of Boran heifers

The objective of the study was to determine whether the presence of CL on the ovary can influence the number and quality of COCs recovered using needle aspiration technique. A total 40 numbers of Bovine ovaries were collected from the local abattoirs soon after the animals were slaughtered. The specimens were classified as corpus luteum present (CL+) and corpus luteum absent (CL) groups. A total of 583 follicles were aspirated from 40 ovaries and among them 381 (62.5%) COCs were collected from CL group ovaries and 202 (37.5%) COCs from CL+ group ovaries. The COCs were also classified as Grade I (>3 layers of cumulus), Grade II (2 or 3 layers of cumulus), Grade III (no cumulus) and Grade IV (degenerated cells). Grade I and Grade II were classified as normal, and Grade III and Grade IV were considered as abnormal COCs. The result indicated that greater numbers of follicles were aspirated and COCs were collected from CL ovaries (15.24±5.27 and 9.40±3.50, respectively) than ovary with CL+. Ovaries CL contribute higher normal COCs (Grades I and II) than that of ovaries with CL+. The result of this study is a preliminary work directing suitable source of COCs for initiating and optimizing in vitro embryo production experiment in like Ethiopia having huge cattle population and animal slaughtering is more common for various reasons like to export or for domestic consumption.