Effect of phosphorus on the growth and photosynthetic pigments content of Helichrysum arenarium (L.) Moench in in vitro cultures

The plant material were plants of sandy everlasting (Helichrysum arenarium (L.) Moench) collected from natural locations in October 2012. Initial explants were apical buds enfolded into two leaves. Isolated explants were chemically sterilized. Reproducted shoots were divided and put into three types of mediums: medium MS (control) containing 37 mg P·dm-3, medium MS without additive of P (0,0 mg P·dm-3), medium MS with additional amound of P (74 mg P·dm-3). The aim of research was to specify the influence of phosphorus content in medium for choosen growth parameters and accumulation of assimilatory pigments: chlorophyll a, b, chlorophyll a+b, carotenoids. Analysis of these compounds were done spectrophotometrically. The made research, proved, that the richest in mentioned substances were microseedlings growing on the medium with increased amount of phosphorus. Phosphorus deficiency in medium MS had a statistically essential effect on changes in growth modifications of microseedlings of sandy everlasting (Helichrysum arenarium (L.) Moench). Additional amount of phosphorus in the medium had a statistically essential effect on increasing of chlorophyll a+b in comparison with plants growing on the medium MS with optimal phosphorus amount or without this element by accordinglly 36% and 23%. Moreover, it caused a growth of content of chlorophyll a, b and carotenoids by accordinglly 18%, 32%, 20% in comparison with variant, where the medium MS without phosphorus was applied.