Interfacing Microchip Based Capillary Electrophoresis System with a Microwave Induced Plasma Optical Emission Spectrometer (uCE-MIP-OES)

A microchip based capillary electrophoresis (uCE) system was interfaced with microwave induced plasma optical emission spectrometry (MIP-OES) to provide rapid elemental separation capabilities. This system uses an extremely low flow micro-cross-flow nebulizer sited directly at the liquid exit of the chip. A supplementary flow of buffer solution at the channel exit was used to improve nebulization efficiency. A small evaporation chamber has been incorporated into the interface in order to prevent the losses associated with traditional spray chambers, allowing the entire sample aerosol to enter the plasma. Syringe pumps were used to manipulate the flow rate and flow direction of the sample, buffer, and supplementary buffer solution. Sample volumes of 40 nanolitre can be analyzed, The feasibility of this hyphenated method for elemental separation was demonstrated by the on-line electrophoretic separation of Ba2+ and Mg2+ ions within 35 s using an 8 cm long separation channel etched in a glass base. Resolution of the Ba2+ and Mg2+ peaks was 0.9 using the chip-based uCE-MIP-OES system.

Opisano metodę rozdziału jonów Ba2+ od Mg 2+ za pomocą mikrosystemu elektroforetycznego (uCE) w połączeniu z optyczną spektrometrią emisyjną (OES) plazmy indukowanej mikrofalowo (MIP). Roztwór próbki (ok. 40 nanolitrów) oraz roztwory buforowe wprowadzano do kanału separacyjnego o długości 8 cm za pomocą pomp strzykawkowych. Zaproponowany system umożliwia rozdział badanych jonów w ciągu 35 s oraz efektywne wprowadzenie próbki, w postaci aerozolu, do źródła wzbudzenia za pomocą układu mikrorozpylacz/minikomora mgielna.