Introduction and objective. The goal of the study was a microbiological, qualitative and quantitative analysis of bioaerosol
at the workplace of medical personnel (Health Emergency Departments (HEDs), ambulances), and comparative administration
offices with an expected neutral occupational exposure to biological agents measured with individual Button Sampler.
Materials and method. Personal sampling was performed with Button Sampler instrument loaded with gelatine filters
in 10 HEDs, in 9 ambulances and in 9 offices to assess the occupational biological agents’ exposure in air. Sampling was
conducted from March until April 2016. Samples were quantitatively assessed for viable and total number of bacteria and
fungi. Routine procedures for microbiological diagnostics were implemented. Data were analysed using Kruskal–Wallis and
Mann-Whitney statistical tests with α=0.05. P value less than 0.05 were considered significant.
Results. At the workplaces assessed, the concentrations of viable microorganisms in HEDs were 1.3 × 102 – 4.2 × 103
CFU/m3 for bacteria, 3.4 × 100 – 8.1 × 101 CFU/m3 for fungi; in ambulances 1.3 × 102 – 1.4 × 103 CFU/m3 (bacteria), 6.7 × 100 –
6.5 × 102 CFU/m3 (fungi) and in offices 4.2 × 101 – 5.0 × 103 CFU/m3 (bacteria), 0 – 7.9 × 102 CFU/m3 (fungi). In outdoor air, the
number of microorganisms reached the level: 1.0 × 102 – 5.9 × 102 CFU/m3 for bacteria and 1.5 × 102 – 8.2 × 102 CFU/m3 for fungi.
The predominant isolated bacteria were Gram-positive cocci. The prevalent fungi species belonged to the genus Aspergillus
and Penicillium.
Conclusions. The quantitative assessment of examined indoor air was similar to control outdoor air, and were relatively
low. The level of microbiological contamination did not exceed 5 × 103 CFU/m3 which is recommended as an admissible
level in public spaces in Poland.
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