Cytogenetic effects of Fusarium mycotoxins on root tip cells of rye [Secale cereale L.], wheat [Triticum aestivum L.] and field bean [Vicia faba L.var.minor]

The effects of fusarial toxins: DAS, T-2, DON, 3 Ac-DON, MON and ZEA, on actively dividing root tip cells of rye, wheat and field bean were investigated. Three concentrations: 1, 5 and 10 µg mL⁻¹ were applied for 24 hours. Nuclei and chromosomes were stained using the Feulgens method. It has been found that trichothecene mycotoxins (DAS, T-2, DON, 3 Ac-DON) had a profound effect on mitosis, as they decreased the mitotic index, produced excessive condensation of pro- and metaphase chromosomes, C-metaphascs and C-anaphases, and caused an accumulation of metaphases. The main effect of trichothecene mycotoxins, probably caused via their influence on protein synthesis, was abnormal functioning of the mitotic spindle.