The Influence of Concentration of Acetic Acid and Pepsin Enzyme in Nilem Fish Skin Collagen Extractionto the Amount of Rendement Produced

The added value of nilem fish skin needs to be increased. The purpose of this study is to determine the concentration of acetic acid solution combined with the pepsin enzyme in the extraction of collagen from nilem fish skin that is necessary to obtain the highest yield/renderment. The study employed an experimental research method that used a completely randomized factorial design. The first treatment is the concentration of acetic acid solution. This consists of three levels, namely 0.5M, 0.7M and 0.9M. The second treatment is the concentration of the enzyme pepsin. This in turn consists of three levels, namely 0.5 %, 1.0 % and 1.5% (weight / weight). The parameters observed were collagen renderment. The results showed that the combination treatment concentration of 0.7 M solution of acetic acid by the pepsin enzyme at 1.0%, in the extraction of collagen from fish skin, produce the highest yield compared to other combinations. The renderment yield is 6.18%.