Partial deglycosylation of α subunit modifies sturgeon gonadotropin function.

Chemical deglycosylation (dg) of sturgeon Acipenser gueldenstaedti Br. (αGTH) resulted in the loss of 83% of its initial carbohydrate content. It altered also recombinant dgαGTH + βGTH dimer molecule, reducing its immunoreactivity by 30%, and fully blocking the hormonal function. CD spectroscopy showed that deglycosylation led to changes in the secondary structure of dgαGTH and in the α-β recombinant. The sugar moiety of sturgeon αGTH is suggested to play an important role in maintaining the biological function of the hormone dimer molecule.