Antimicrobial Potency from Endophytic Bacteria of Bignay Plant (Antidesma bunius (L.) Spreng.) Against Pathogenic Bacteria

The need for new drugs is increasing in the antibiotic field. However, synthetic antibiotics often have a bad impact on body health, hence, in this paper, endophytic bacteria is investigated as an antibiotic source. The purpose of this research is to gain an understanding of the antibacterial potency of the endophytic bacteria associated with the Bignay plant (Antidesma bunius) against pathogenic bacteria that is Gram-negative i.e Salmonella thypimurium ATCC 14028, Escherichia coli ATCC 35218 and Gram-positive i.e Staphyllococcus aureus ATCC 6538. This research used the descriptive method and consists of endophytic bacterial isolation, identification, and antimicrobial testing. The endophytic bacterial isolation from fruit and stem of Bignay plant was performed by using the pour plate method, while the bacteria was identified by applying a biochemical test method using Vitek 2.0. The antimicrobial test was performed by way of paper disc diffusion. The resulting endophytic bacteria isolated from the fruit and stem of the Bignay plant are Bacillus pumilus, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus sp.1, and Bacillus sp.2. The most potential endophytic bacteria as antimicrobial against pathogenic bacteria were found to be Bacillus pumilus, Bacillus sp.1, and Bacillus sp.2.