Ocena wystepowania Pneumocystis carinii u chorych zakazonych wirusem HIV w oparciu o wyniki trzech metod diagnostycznych

The frequency of Pneumocystis carinii occurrence in BAL of 38 HIV-infected patients was determined with three different method. BAL sediments were stained with Giemsa method, silvered according to Gomori-Grocott method and studied with indirect immunofluorescence assay. Using Giemsa method staining Pneumocystis carinii was diagnosed in 81,6% of patients, in Gomori-Grocott method - in 31,6% of patients, but results of indirect immunofluorescence assay were positive only in 23,7%. In our study staining BAL sediments with Giemsa method allowed to detect Pneumocystis carinii in the highest percentage of examined patients.