In this study, milk samples were taken in sterile 20 ml containers from 90 cows with clinical
mastitis in various enterprises around Aydın province between May-September 2017. The
presence of Aspergillus flavus M1 toxin in milk samples was investigated by ELISA. Aflatoxin
M1 ELISA results were compared with positive controls with 0 ppt, 5 ppt, 15 ppt, 30 ppt, 60
ppt and 100 ppt. For the 88 samples examined; 5 ppt in 7 (8,0 %), 15 ppt in 12 (13,6 %), 100
ppt in 32 (36,4 %) and 0 ppt in 37 (42,0 %) aflatoxin M1 was detected. According to these
results, 32 (36,4 %) AFM1 positivity was detected in the European Union countries over the
limit (50 ppt) accepted for raw milk. In our study, DNA obtained from 88 mastitis milk samples
examined with Aflatoxin M1 ELISA kit was subjected to Aspergillus flavus species specific
PCR process. As a result of PCR; Aflatoxin M1 at a level of 0 ppt in 7 (36,8 %), 5 ppt in 1 (%
5,2), 15 ppt in 2 (10,5 %) and 100 ppt in 9 (47,5 %) ELISA kit toxin presence was determined.
As a result, 32 samples of 100 ppt AFM1 positivity were detected by ELISA and 9 samples 100
ppt positivity was detected by PCR in our study. The results of the ELISA test revealed that
more positive results were formed due to cross-reactions, and the PCR method with FLA gene
primers was more reliable for diagnosis.
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