CellProfiler and WEKA Tools: Image Analysis for Fish Erythrocytes Shape and Machine Learning Model Algorithm Accuracy Prediction of Dataset

The first part of the study was detected the number of cells and measurement of shape of cells, cytoplasm, and nuclei in an image of Giemsa-stained of fish peripheral erythrocytes by using CellProfiler (CP, version 2.1.0) tool, an image analysis tool. In the second part, it was evaluated machine learning (ML) algorithm models viz. BayesNet (BN), NaiveBayes (NB), logistic regression (LR), Lazy.KStar (K*), decision tree (DT) J48, Random forest (RF) and Random tree (RT) in the WEKA tool (version 3.8.5) for the prediction of the accuracy of the dataset generated from an image. The CP predicts the numbers and individual cellular area shape (arbitrary unit) of cells, cytoplasm, and nuclei as primary, secondary, and tertiary object data in an image. The performance of model accuracy of studied ML algorithm classifications as per correctly and incorrectly classified instances, the highest values were observed in RF and RT followed by K*, LR, BN and DTJ48 and lowest in NB as per training and testing set of correctly classified instances. In case of performance accuracy of class for K value, the highest values were observed in RF and RT followed by K*, LR, BN and DTJ48 and lowest in NB while lowest values were obtained for mean absolute error (MAE) and root mean squared error (RMSE) in case of RT followed by RF, K*, LR, BN and DTJ48 and comparatively highest value in case of NB as per training and testing set. In conclusion, both tools performed well as an image to the dataset and obtained dataset to rich information through ML modelling and future study in WEKA tool can easily be analysed many biological big data to predict classifier accuracy.