Extremely halophilic diversity of IncheBroun wetland located in the north of Iran was
investigated by using culture-dependent methods. Sampling was carried out in May and September
2014. In each sampling 4 distinct regions of wetland were analyzed by using complex media like
MGM, JCM168, MH1 and an alkaliphilic medium containing 23% salts. After incubation at 40˚C, a
total of 406 isolates and 2.1 × 106 CFU/ml were obtained in culture media. Among them
361 isolates were obtained from MGM and 39 isolates from JCM 168, 3 isolates from MH1 and
3 isolates from the alkaliphilic media. Initial morphological, biochemical and physiological tests
were performed. Production of 4 hydrolytic enzymes by 45 selected strains was assayed
qualitatively. A total of 38, 19 and 6 strains were able to produce lipase, DNase and amylase
activity. Protease activity was not observed among strains. As total 45 strains were selected
randomly and phylogenetic analysis of 16S rRNA was performed for them. Among selected strains
40 isolated strians belonged to Haloarchaea and were belonged to the genera: Haloarcula(30%),
Halorubrum(27.5%), Haloferax(17.5%), Halobellus (10%), Halogeometricum(5.2%),
Halobacterium(2.6%), Halolamina(2.6%), Halorhabdus (2.6%) and Halostagnicola (2.6%).
Haloarcula and Halorubrum were the dominant populations. A total of 5 strains belonged to
domain of Bacteria and were similar to members of Rhodovibrio (40%), Pseudomonas (40%) and
Salicola (20%).
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